Overview Data used for manuscript: Temnothorax rugatulus ants do not change their nest walls in response to environmental humidity Structure of the data SupplementalHygrometerDatabase.csv Raw hygrometer data that is used to calculate the average environmental humidity and temperature for each Trial:Salt combination Colony: Unique experimental colony identifiers TrialNumber: Sequential trial number (1-4) that is NOT unique for each colony - see "Trial" Salt: Saturated salt solution used "Date Time, GMT-07:00": Date and time of each observation Temp: Temperature in celcius RH: Relative humidity (%) Trial: The trial number for each individual colony, each colony underwent two trials HumidityExperimentalDatabase.csv Raw experimental data with nest features and colony size Colony: Unique experimental colony identifiers Trial: The trial number for each individual colony, each colony underwent two trials TrialNumber: Sequential trial number (1-4) that is NOT unique for each colony - see "Trial" Day: The day in the experimental timeline (always 10, but days 1 and 5 were captured and not considered) Area: Area of the built nest wall (mm2) Length: Length of the built nest wall (mm) Nest.Area: Area of the internal nest space (mm2) HumLevel: Whether the colony started with a higher or lower relative humidity (High/Low) Number.Ant: The number of workers in the colony Number.Brood: The number of brood in the colony Number.Queens: The number of brood in the colony Salt: Saturated salt solution used SubstrateISide: Substrate I placement in the container from the perspective of looking out from the nest entrance StartWtI: The initial weight (g) of the available substrate I building nest wall material UsedWtI: The weight (g) of the available substrate I building nest wall material following the experimental building phase StartWtII: The initial weight (g) of the available substrate II building nest wall material UsedWtII: The weight (g) of the available substrate II building nest wall material following the experimental building phase CollWallWt: The weight (g) of the experimental nest wall that each colony built HumidMortalityRaw.csv Raw experimental data with proportion of workers and brood dead after each colony underwent Trial 1 Colony: Unique experimental colony identifiers WorkerDeath: Proportion of workers that died BroodDeath: Proportion of brood that died TrialNumber: Sequential trial number (1-4) that is NOT unique for each colony PorosityComparisonRaw.csv Data used for comparing the porosities of each experimental substrate, experimentally built walls, and collected Temnothorax rugatulus walls Porosity is the percentage of void space in compact substrate - PoreVolume/TotalVolume SubstrateID: A unique identifier for each substrate replicate Trial: The trial number for each individual colony, each colony underwent two trials (only applicable for experimentally built walls) SubCategory: The type of substrate (Sub I, Sub II, Built, Natural) TotalVolume: The combined pore (void space in compact substrate grains) and soil volume (ml) of a substrate PoreVolume: The void space in between compact substrate grains (ml)

Funding for this project was provided by: NSF grants DGE-1746060 to GC, IOS-3014230 and ABI-3019760 to AD