In this study, four sampling points from the major river that flows through Akure, Ondo State, Nigeria was considered. A total of 16 samples were obtained from the sampling points and some physico-chemical and bacteriological tests were carried out on each sample. Statistical analysis of the water parameters was carried out using SPSS statistics 22 software, and a comparison between the mean value of the parameters in each location to the World Health Organization (WHO) standard was made for each water quality parameter. The results obtained from the statistical analysis showed that of all the physico-chemical and bacteriological parameters tested only two parameters (sulphate and zinc) had a P value that was > 0.05 which implies that they did not significantly affect surface water quality in the study area, while other parameters fell short of this criterion. It is recommended that water from these surface water sources undergo adequate treatment before potable use.