Mica has been proven to have anticancer effects, however, there is not much data available on its efficacy and treating mechanism. In Ayurveda- an Indian system of medicine, a medicine named ‘Abhrak bhasma’, is incinerated mica-based nano-material being used for centuries to treat various respiratory conditions and lungrelated diseases. Nanoparticles are proven to have advantages in cancer treatment. The purpose of this study was to examine ‘ Abhrak Bhasma’ induced cytotoxicity on the A549 cell line. Method - Cytotoxicity was measured by MTT assay on A549 cell line Result - Mica nanoparticles at up to 200µ/mL for 24 hours showed concentration dependant cytotoxic activity. The calculated IC50 value was 168.60µg/mL Conclusion - This is the first report showing that Abhrak bhasma or mica nanoparticles induce cytotoxicity in A459 cells.