Slimfield: Escherichia coli DNA repair proteins (RecA-mGFP and RecB-sfGFP)

Research datakeyboard_double_arrow_right Dataset 13 Jun 2022 English Publisher:ZenodoFunded by:UKRI | Biological physics of pro..., UKRI | Replication repair in rea..., UKRI | Pushing proteins off DNA ...

Authors: Payne-Dwyer, Alex L; Syeda, Aisha H; Leake, Mark C;

doi: 10.5281/zenodo.6639101 , 10.5281/zenodo.6639100

Slimfield: Escherichia coli DNA repair proteins (RecA-mGFP and RecB-sfGFP)

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Abstract

Imaging modality / instrument: Brightfield + Slimfield Image format: OME TIFF (16 bit) + MicroManager metadata files Microscope settings: 488 nm triggered excitation; split detection, cropped to GFP or RFP/GFP (left/right) channels; 3 ms/frame laser exposure; Photometrics Prime95b CMOS Samples and acquisitions: Fluorescent fusions in live E.coli cells. MMC = mitomycin C No. fields of view MMC- MMC+ (0.5 ug/ml 3h) RecA-mGFP 7 15 RecB-sfGFP 17 21 MG1655 control 10 10 Approx. size before/after compression: 21 GB / 7 GB

{"references": ["\"RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live Escherichia coli with single-molecule sensitivity\", Alex L. Payne-Dwyer, Aisha H. Syeda, Jack W. Shepherd, Lewis Frame, Mark. C. Leake bioRxiv 2021.10.04.463068; doi: https://doi.org/10.1101/2021.10.04.463068"]}

Related Organizations

University of York
United Kingdom

Keywords

single-molecule imaging, DNA repair, Slimfield, bacteria, fluorescence microscopy

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