This deliverable describes several statistical approaches that provide insights into real-life mixtures. In addition, it provides statistical methods that can be used to gain insight into the determinants of mixture profiles in HBM data. The deliverable provides practical instructions on how to apply the statistical methods using the R language. At each step we provide a demonstration application using a dataset that was simulated to represent a real-life example of HBM data. A brief description of the potential inferences that can be made when applying the methods on real-life data is also provided.