Fluorescence labeling of tumors allows surgeons to better delineate anatomic structures in real time, as there is immediate feedback while the tissue is being manipulated. In addition ,Fluorescence-guided surgery (FGS) can potentially be used during all colorectal surgeries, especially for deep or locally-aggressive colon cancers, rectal cancers, cancers after neoadjuvant treatment, and cancers where an organ-sparing approach is being utilized (i.e., submucosal or full-thickness resections). ICG is the most commonly used molecule in the field of fluorescence-guided surgery and has gained widespread popularity for a wide variety of NIR fluorescence applications. So encapsulating of ICG into nanoparticles (NPs), for example PLGA NPs, results in the extension of ICG half-life and accumulation at the tumor site.