Protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (IFT) in C. elegans

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 2022 Turkey English Publisher:Elsevier BVJournal:STAR Protocols, volume 3, page 101,498 (issn: 2666-1667,

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Authors: Merve G. Turan; Hanife Kantarci; Sadiye D. Temtek; Onur Cakici; Sebiha Cevik; Oktay I. Kaplan;

doi: 10.1016/j.xpro.2022.101498 , 10.5281/zenodo.6581942 , 10.5281/zenodo.6568214 , 10.5281/zenodo.6568213 , 10.5281/zenodo.6582147

pmid: 35776634

pmc: PMC9250072

handle: 20.500.12573/1315

Protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (IFT) in C. elegans

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Abstract

These videos were included in the paper "Protocol for determining the average speed and frequency of kinesin and dynein driven Intraflagellar Transport (IFT) in C. elegans." In addition to wild-type movies that were used to determine both the speed and frequency of IFT, triple mutants wdr-31(tm10423)II;elmd-1(syb630) III; rpi-2(ok1863)IV. [GOU2362, ift-74 (cas499 [ift-74::gfp]) II.] were only used to determine the frequency in the paper.

In files N2 [IFT-74GFP] represents wild-type, additively wdr-31(tm10423)II;elmd-1(syb630) III; rpi-2(ok1863)IV. [GOU2362, ift-74 (cas499 [ift-74::gfp]) II.] represents triple mutants used in protocol.

Country

Turkey

Related Organizations

Abdullah Gül University
Turkey
KAYSERI UNIVERSITY
Turkey

Keywords

Microscopy, Science (General), Bioinformatics, Dyneins, Kinesins, Cell Biology, Q1-390, #cilia #IFT #celegans, Model Organisms, Flagella, CRISPR, Mutation, Genetics, Protocol, Animals, Cilia, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Molecular Biology, Neuroscience

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