Our research identified 12 species of naked amoebae of eight morphotypes in the Black Sea (coastal zone of Zatoka village, Odessa region). In more than half of the samples, Vannella devonica, Vannella aberdonica and Thecamoeba orbis were present. The species Saccamoeba marina, Vexillifera armata, Vannella plurinucleolus, Cochliopodium gulosum, and Stenamoeba sp. were rare and infrequent in our samples. The upper layers of the bottom sediment were inhabited by all 12 species of amoebae; two layers were inhabited by S. marina, V. armata, V. devonica, V. aberdonica, Mayorella gemmifera, T. orbis, Stenamoeba sp., Acanthamoeba griffin; three layers were colonized by V. armata, V. devonica, V. aberdonica, T. orbis, Stenamoeba sp., and A. griffini. All amoebae species and their morphotypes occurred at Black Sea water temperatures of +22 ºС to +26 ºС and salinity of 15.5 ‰ to 17.6 ‰. The species V. simplex and A. griffini, which belong to the fan–shaped and acanthopodial morphotypes, respectively, were collected in samples from the Mediterranean Sea at water temperatures of +29 ºС and salinity of 37.8 ‰. The amoebas identified belong to two classes, seven orders, eight families and eight genera.