extHomFam 2 was constructed by combining Homstrad reference alignments (March 2020) with Pfam 33.1 complete families (NCBI variant). Homstrad entries with less than 3 reference sequences and those pointing to dead Pfam families were discarded. The resulting benchmark was divided into subsets depending on the family size N: subset N range # families small [200, 10 000) 86 medium [10 000, 40 000) 95 large [40 000, 100 000) 83 xlarge [100 000, 250 000) 67 huge [250 000, 3 000 000) 62 The directories in the archive correspond to the names of the subsets, while the reference alignments are located in 'ref' folder.

{"references": ["Deorowicz, S., Debudaj-Grabysz, A. & Gudy\u015b, A. FAMSA: Fast and accurate multiple sequence alignment of huge protein families. Sci Rep 6, 33964 (2016). https://doi.org/10.1038/srep33964"]}