Introduction Location of tumour within the colon is gaining traction as a crucial factor in determining disease progression, prognosis and management. Clinicopathological features, protein / genetic biomarkers, and response to therapy, varies between left (LSCC) and right sided colon cancer (RSCC).[1] However, differences in tumour microenvironment, particularly the distribution and density of extracellular matrix (ECM) has not been studied. We used 2-photon laser scanning microscopy (2PLSM) to visualise label-free components of ECM, so as to depict specific patterns in LSCC and RCSS for subclassification. References [1] Mukund, K., Syulyukina, N., Ramamoorthy, S., & Subramaniam, S. (2020). Right and left-sided colon cancers-specificity of molecular mechanisms in tumorigenesis and progression. BMC cancer, 20(1), 1-15. [2] Wershof, Esther, et al. "A FIJI Macro for quantifying pattern in extracellular matrix." Life science alliance 4.3 (2021). [3] Elsaleh, H., Joseph, D., Grieu, F., Zeps, N., Spry, N., & Iacopetta, B. (2000). Association of tumour site and sex with survival benefit from adjuvant chemotherapy in colorectal cancer. The Lancet, 355(9217), 1745-1750.