Green snow microorganisms play an important role in biogeochemical cycle and carbon sink processes and they can be a source of biotechnologically interesting cell factories. A wide temperature tolerance is a unique property of bacteria isolated from cold environments that has received great attention in the last years. The present paper examines the growth and chemical profile flexibility for green snow bacteria exposed to different temperature and nutrient fluctuations. By applying high-throughput chemical phenotyping with FTIR spectroscopy we discovered chemical changes possessed by green snow bacteria when grown at high/low temperature and rich/minimal media.