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ZENODO
Other literature type . 2022
License: CC BY
Data sources: ZENODO
ZENODO
Other literature type . 2022
License: CC BY
Data sources: Datacite
ZENODO
Other literature type . 2022
License: CC BY
Data sources: Datacite
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Production of Recombinant α Synuclein Monomers and Preformed Fibrils (PFFs)

descriptionPublicationkeyboard_double_arrow_right Other literature type 09 Apr 2022 English Publisher:Zenodo
Authors: Manecka, Destiny-Love; Luo, Wen; Krahn, Andrea; Del Cid Pellitero, Esther; Shlaifer, Irina; Nicouleau, Michael; Pimentel, Luisa; +3 Authors

doi: 10.5281/zenodo.3372887 , 10.5281/zenodo.6430401

Production of Recombinant α Synuclein Monomers and Preformed Fibrils (PFFs)

Abstract

This protocol describes how to: • Produce and purify recombinant human and mouse α-synuclein in monomeric form from transformed bacteria • Generate α-synuclein preformed fibrils (PFFs) from recombinant monomeric α-synuclein • Fluorescently label α-synuclein PFFs Version 3.0 now available

Related Organizations
Keywords

alpha-synuclein, GST-tag, Preformed fibrils (PFFs), bacteria, protein expression

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download
selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
OpenAIRE UsageCountsViews provided by UsageCounts
downloads
OpenAIRE UsageCountsDownloads provided by UsageCounts
6
Top 10%
Average
Top 10%
46
48
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