This is an updated version of our original data archive (which can be found under the doi 10.5281/zenodo.5513025) that reflects changes we've made to the manuscript over the course of the review process and incorporates the new data we've collected since the time of the initial bioRxiv submission. This data archive contains all raw data EXCEPT for single-particle microscopy movies (which are deposited separately due to their size) for our paper titled "Endoplasmic reticulum stress activates human IRE1a through reversible assembly of inactive dimers into small oligomers". These raw data are stored in "non_SPT_data_final_v2.zip". Additionally, full plasmid sequences for all plasmids used in this paper are stored in GenBank format in the file "plasmid_sequences_v2.zip". Finally, this repository contains processed single-particle movies in the form of dual-color tracks from the TrackMate ImageJ plugin in XML format (file: "SPT_processed_data_and_settings_final_v2.zip"). The processed XML tracks are organized in the same way as the raw data files in the separate repository. They are sorted into subfolders by date of acquisition first, followed by experimental conditions. To recreate the figures from the paper, follow instructions in the README.md file included with the source code repository and use the JSON settings files saved here under "analysis_settings".