A recent study of patients undergoing implant surgery showed a significant increase in the prevalence of peri-implant disease, with peri-implant mucositis and peri-implantitis predominating. Peri-implant disease mostly appears after several years of implant use. Currently, the diagnosis of peri-implant disease relies on peri-implant soft tissue exploration and imaging, but abnormalities in these indicators only occur when tissue destruction has already occurred and cannot predict whether an implant has the potential to develop peri-implant disease. Therefore, the early diagnosis of peri-implant disease remains challenging and early diagnosis is important to achieve a good prognosis. Gingival crevicular fluid is a biological exudate present in the gingival crevicular, which is mainly derived from serum and contains metabolic components of bacteria and host cells. Gingival crevicular fluid is considered to be an important diagnostic material and its biomarkers can be used as reaction indicators to determine the development of the disease. In this paper, we describe the progress and effectiveness of research on the use of gingival crevicular fluid biomarkers for the prediction and diagnosis of peri-implant disease, intending to provide a reference for subsequent studies.