The public cell line collection of the Human and Animal Cell Line department at DSMZ today holds more than 860 cell lines available for the research community. Of these about 600 are of human origin and about 260 of animal origin. Human cell lines are mostly derived from malignant tumour material donated by patients. Cell lines serve as in vitro models for the respective diseases and are of importance for understanding the molecular changes underlying human disease. In the past 10 years high-throughput sequencing techniques became available and enabled in depth analysis of the cellular genome and transcriptomes. In 2016 we started to perform RNA-seq of all human cell lines included in the open cell line collection. DSMZCellDive offers analysis tools like barplots and heatmaps to plot gene expression leves in cell lines of choice, which are presented according to cell type/tumour entity. This enables researchers to decide on the best appropriate model for their research questions.