qudi-HiM is a software package developed by the Nollmann Lab at the Center of Structural Biology, a department of the CNRS and the INSERM. qudi-HiM is a tool for the control of optical microscope setups with different hardware components optimized to performed multiplexed imaging experiments. qudi-HiM has been build using a modular approach so that it can be adapted to different microscopes with various optical modalities (e.g. widefield/ confocal) and hardward components by using local configuration files. The graphical user interface (GUI) was build using pyQt. qudi-HiM is built using the framework provided by Qudi, a suite of tools for operating multi-instrument and multi-computer laboratory experiments. The Qudi Github repository contains a detailed documentation for users as well as guidelines for contributors.