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We use the five-sixths nephrectomy (5/6 Nx)-induced CKD rats to investigate whether TMAO aggravates kidney damage and its possible mechanisms. Six weeks after the operation, the two groups of 5/6 Nx rats were subjected to intraperitoneal injection with 2.5% peritoneal dialysis fluid and 2.5% PDF plus TMAO 20mg/kg/d.
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