The datas correspond to article entitled: "Denaturing and dNTPs reagents improve SARS-CoV-2 detection via single and multiplex RT-qPCR". The file entitle GISAID have the fasta formats for 107259 genomes from the SARS-CoV-2 GISAID database from January to December 2020. Three documents in plane tex correspond: sequences.fasta contain the original data. sequences_clean.fasta. Corresponds to genomes sequences without nucleotides undeterminateds indicates with "N" in previous document. alignment_clean.fasta. Contain the genomes sequences cleaned alingment. The file entitle GenBank have the data set from 19317 genomes from the SARS-CoV-2 GenBank database from January to October 2020 and the documets have the prrevious order.

These data are also associated with the national project entitled: "Contribution to the diagnostic protocol of the Coronavirus SARS-CoV-2 and the Influenza A H1N1 Virus by real-time RT-PCR in one step authorized by the World Health Organization with the inclusion of marker genes required for viral infection and their validation by next-generation genomic sequencing with nucleotide concentration technology" financed by the Vice-Rectory of Research and Extension of Industrial University of Santander [project No. 76900] and the Ministry of Science, Technology and Innovation (MinCiencias) of Colombia [No. 1015-2020 - Mincinciaton].