Reference datasets (July 2022 update) for Mitohelper (https://github.com/aomlomics/mitohelper) Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline. I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information: mitofish.all.Jul2022.tsv (748,772 records) mitofish.12S.Jul2022.tsv (40,541 records) mitofish.COI.Jul2022.tsv (265,763 records) II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets: 12S-seqs-derep-uniq.qza 12S-tax-derep-uniq.qza 12S-16S-18S-seqs.qza 12S-16S-18S-tax.qza If you use Mitohelper, please cite: Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10. https://doi.org/10.1002/edn3.187

In the July version of Mitofish, 6,440 UNVERIFIED records were included. These were removed in mitohelper's dataset. In addition, 654 valid records were in old versions of Mitofish but not in the July version. These were not removed in mitohelper's dataset.