In this scientific paper the authors considered the challenges that developing countries experience when participating in the Dispute Settlement Body (DSB) of the World Trade Organization (WTO). Developing countries and the low-income countries are faced with limitations of the DSB and, therefore, this research examines the most substantial aspects of these limitations. The current research article discusses the economic, financial and legal constraints encountered by developing countries and the least developed countries. This research also explains the need to ameliorate the Dispute Settlement Understanding (DSU).