To study the Physico and phyto chemical characters of the plant Mimusops elengi L (family Sapotaceae) and also to carry out the thin layer chromatographic fingerprint. Phytochemical investigations and fluorescence analysis were carried out as per the standard techniques. Various quantitative parameters like ash values, extractive values and flavonoid content can be used as quality control parameters for the plant Mimusops elengi L were determined. The ethanolic extract with ethanol and analysis was developed by using Thin Layer Chromatography (TLC) technique equipped with pre coated Silica plate of 12cm height using automatic TLC scanner. Alkaloid, tannin, flavonoid, cardiac glycosides, and coumarins were found in preliminary phytochemical research. TLC examination of an ethanolic extract of Mimusops elengi L revealed a variety of phytoconstituents with varying mobile phase compositions. The plant's TLC fingerprint was examined. Mimusops elengi L is helpful as a phytochemical marker and an excellent estimator of genetic variability in plant populations, and it can be used as a diagnostic tool. Keywords: Plant extract of Mimusops elengi L leaf, Phytochemical screening, fluorescent character, TLC analysis.