Supplementary materials of Brain Extract of Subacute Traumatic Brain Injury Promotes Neuronal Differentiation of Human Neural Stem Cells Via Autophagy
Supplementary materials of Brain Extract of Subacute Traumatic Brain Injury Promotes Neuronal Differentiation of Human Neural Stem Cells Via Autophagy
Figure S1: Characterization of hNSCs. In proliferate medium, the embryo derived hNSCs could form the neurospheres (A). Markers of NSCs (NES, SOX2, SOX1) were detected (B). The SOX2+ cells accounted for >95% of all cells (C). Bar scale: 60μm; Figure S2: The proliferation of hNSCs in TBI brain extracts with different phases. Differentiated hNSCs could be labeled with EdU in TBI brain extracts with different phases (A). Ratio of EdU+ cells was decreased with the progression of days post-injury (B). Bar scale: 75μm; ****: P<0.0001; Figure S3: The GO annotations and KEGG pathway analysis of different expressed pro-teins in acute brain extract and subacute brain extract; Table S1: The protein profiles of brain extract in acute phase and subacute phase after TBI.
- Shanghai Jiao Tong University China (People's Republic of)
- Renji Hospital China (People's Republic of)
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