Aim: To assess the association of vitamin D status in preeclamptic and normal healthy pregnant women. Materials and Methods: Prospective case control study included 50 women with preeclampsia and 50 normotensive pregnant women. Serum vitamin D estimation was done. Correlation between vitamin D deficiency and preeclampsia was analysed statistically. Results: Prevalence of vitamin D deficiency was comparable in both groups. Mean vitamin D level (19.7±2.79 ng/ml) was statistically low in preeclampsia group while in that of control was 24.3±1.89 ng/ml. Severity of vitamin D deficiency correlated with the severity of preeclampsia. Age, parity and BMI were similar in both the groups. No statistically significant difference seen regarding mode of delivery in both the groups. Conclusion: Vitamin D deficiency is significantly associated with preeclampsia. Further studies are needed to document the role of vitamin D supplementation in prevention of preeclampsia.