This dataset provides the data needed for the Galaxy BASIC assembly workflow training tutorial (https://galaxy-synbiocad.org). This workflow provides a pathway to design plasmids encoding predicted metabolic pathways using the BASIC assembly method (https://doi.org/10.1021/sb500356d). It generates scripts allowing the automatic construction of these plasmids using an Opentrons liquid handling robot. After downloading these scripts on a computer connected to an Opentrons (https://opentrons.com), the user can perform the automatic construction of the plasmids on the bench. The content of the dataset is as follows: an SBML file modeling a heterologous pathway producing lycopene such as those produced by the Pathway Analysis Workflow (https://galaxy-synbiocad.org). a CSV file listing the parts to be used (linkers, backbone and promoters) in the constructions. two YAML files providing two examples of settings, i.e. providing the identifiers of the laboratory equipment and the parameters of the DNA robot.