Pathogenic strains of Escherichia coli (E. coli) are a major threat to human health causing foodborne and waterborne diseases from gastrointestinal symptoms and diarrhea to even fatal hemolytic uremic syndrome. Enterobactin (Ent) is the principal siderophore secreted by E. coli, important in bacterial growth, replication and infection process, being also electrochemically active. Its rapid detection could allow the development of portable devices for on-field testing of bacteria contamination with applications in environmental, industrial and clinical settings.