Nanoparticles are small particles with a diameter of 10 to 1000 nanometers. The extract of Cirrhinus mrigala was utilised to make nanoparticles. The colour altered from light color to dark brown color, showing the first appearance of AgNPs (i.e. silver nanoparticles). The nanoparticles were filtered by 15,000 rpm centrifugation for 30 minutes. Antibacterial activity was carried out three pathogens were used Pseudomonas aeruginosa, Klebsiela pneumonia and Staphylococcus aureus it was concluded that C. marigala AgNps showed stronger bactericidal activity compared C. marigala aqueous extract. The highest significant effective antibacterial was shown by AgNPs and extract of C. marigala against Pseudomonas aeruginosa (24 mm ± 0 mm and 21 mm ± 0 mm respectively). The principle component analysis (PCA) first two axes explained 91.375 of the variation in the sampled avian community (Component 1: 76.311 percent; Component 2: 15.064 percent).Variables loading onto Component 1 included AgNP-1 (r = 5.8427), AgNP-2 (r = -0.74325), AgNP-3 (r = 0.29089), Ext-1 (r = -1.8331), Ext-2 (r = -1.6154) and Ext-3 (r = -1.9419). AgNP and extract also loaded onto component 2 (AgNP-1: r = -0.67209; AgNP-2: r = 1.8906; AgNP-3: r = 1.5096 and Ext-1: r = -0.90355; Ext-2: r = -0.93844 and Ext-3; -0.8861).