Data underlying the figures in the publication “Rational design and construction of multi-copy biomanufacturing islands in mammalian cells”, published in Nucleic Acids Res., 2022, 50(1), 561-578. DOI:10.1093/nar/gkab1214 Table of contents: 1. tableSx.zip: 8 .zip archives containing the raw data for the Tables S1-S8. 2. bioIslands_Supp_Figs_Info_Tables.pdf Data Availability Statement DNA sequences specifying all coding and regulatory elements used to assemble our multiple gene constructs are provided in the Supplementary Data. Novel sequences have been deposited in the NCBI database. Accession numbers are provided in Supplementary Tables S2-S7. The gene diversifier and optimizer software is freely available for download at https://github.com/altamurr/Gene-Diversifier. Flow cytometry data has been deposited in FlowRepository. Links to FlowRepository are available in the section “Flow Cytometry Data” in the Supplementary Data. Microbial and mammalian strains as well as plasmid constructs used to complete this study are available under a Material Transfer Agreement.