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Article . 2014
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Article . 2014
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SCREENING TEST FOR DETECTION OF COLORADO POTATO BEETLE (Leptinotarsa decemlineata Say ) SENSITIVITY TO ABAMECTIN

Authors: SLAVICA VUKOVI��; DU��ANKA IN��I��; SONJA GVOZDENAC; SNE��ANA TANASKOVI��;

SCREENING TEST FOR DETECTION OF COLORADO POTATO BEETLE (Leptinotarsa decemlineata Say ) SENSITIVITY TO ABAMECTIN

Abstract

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Screening test was carried out to assess the sensitivity to 19 filed populations of Colorado potato beetle (Leptinotarsa decemlineata Say.) to abamectin during 2008 (Futog, ��enej, Vilovo, Kovilj and ��urug), 2009 (Stepanovi��evo, Glo��ane, ��abalj, Sirig, ��ajka��, Ljutovo, ��imu��e, Ba��ko Gradi��te, Novo Milo��evo i Kikinda) and 2012 (Zmajevo, Despotovo, Vilovo and ��urug). The test provided a quick assessment of sensitivity of overwintered adults to abamectin (commercial insecticide Abastate) applied at label rate (0.75 l/ha), two-fold (1.5 l/ha) and five-fold higher rates (3.75 l/ha), by soaking method (5 sec). Mortality was assessed after 72 h of exposition. Results were corrected for the mortality in the control and presented as efficacy (%) after the treatment. According to IRAC (method 011), with slight corrections, the sensitivity of Colorado potato beetle was classified by scale 1-5 (1 - highly sensitive population (E= 100%); 2 - sensitive (100 > E ��� 95%); 3 ��� slightly resistant (95>E ���90%); 4 - resistant (90>E ���50%); 5 ��� highly resistant (E < 50%). From five tested populations in 2008, four were highly sensitive (E=100%) to abamectin at label rate and one was sensitive (E=97.5%). In 2009, out of ten tested populations, three were slightly resistant (E=92.6-94.6%), five were resistant (E=64.6-88.7%) and two were highly resistant (E=22.5-48.3%) to abamectin at label rate. Out of four tested populations in 2012, one was highly sensitive (E=100%), one was sensitive (E=95.8%) and two were slightly resistant (E=90.6-94.2%). The survival of adults in treatments with higher rates of abamectin, that the label one, prove that there is a part of population which has endured changes in susceptibility or selection to resistance.

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Keywords

Colorado potato beetle, abamectin, sensitivity, screening test

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