Department of Chemistry, S. G. R. Post Graduate College, Dobhi-222 149, Uttar Pradesh, India (Purvanchal University, Jaunpur-222 001, Uttar Pradesh, India) Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221 005, Uttar Pradesh, India Manuscript received 18 February 2005, revised 29 November 2005, accepted 22 December 2005 Three navonoids, 4,7-dimethoxyisoflavone, quercetin and kaempferol-4'-methyl ether have been isolated from the whole plant of Rhamnus nipalensis and their structures established by spectral evidences. This is the first report of these flavonoids in R.\( nipalensis.\)