Department of Chemistry. University of Rajasthan. Jaipur-302 004, India E-mail : kudiwal@datainfosys.net Fax: 91-141-2708621 Civil Engineering Department. MNIT. Jaipur, India Manuscript received 23 September 2004, revised 2 June 2005, accepted 21 July 2005 The results obtained In the present Investigations of NO3-N In the effluent was found to he lower than the tolerance limit 10 mg/L as NO3-N prescribed by the WHO. Hence, when glucose was used as a carbon source at C/N ratio of 2.5, the organics started entering In the treated water. The sodium acetate is cheapest than other carbon sources. The present work provides a better alternate source of carbon for in situ denitrification through Pseudomonas stutzeri.