This paper introduces the segmentation of Neisseria bacterial meningitis images. Images segmentation is an operation of identifying the homogeneous location in a digital image. The basic idea behind segmentation called thresholding, which be classified as single thresholding and multiple thresholding. To perform images segmentation, transformations and morphological operations processes are used to segment the images, as well as image transformation an edge detecting, filling operation, design structure element, and arithmetic operations technique is used to implement images segmentation. The images segmentation represent significant step in extracting images features and diagnoses the disease by computer software applications.