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Cepa C924 fluorescente mediante marcaje molecular para estudios de mecanismos de acci��n

Authors: Dominguez Rabilero, Laritza; D��az Gonz��lez, Omar; Nordelo, Aylin; S��nchez Ortiz, Ileana; Franco Rodr��guez, Ram��n; Somonte S��nchez, Danalay; Carrazana Granado, Dulemy;

Cepa C924 fluorescente mediante marcaje molecular para estudios de mecanismos de acci��n

Abstract

En el CIGB Camag��ey, Cuba, se aisl�� a partir de la rizosfera de plantas la cepa bacteriana C924, clasificada posteriormente como Brevibacterium celere. Esta cepa mostr�� potencialidades como estimulador del crecimiento vegetal, as�� como en el control biol��gico de nematodos. Sin embargo, se establece como necesidad dilucidar el mecanismo de acci��n de esta cepa en la interacci��n que establece con las plantas. Para lograr entender este fen��meno se propone como estrategia la construcci��n de un vector que porte el gen reportero de la prote��na roja fluorescente DsRed para marcar la cepa C924, como herramienta de estudio. Con este fin se emplearon los pl��smidos: pTNR-KA como receptor de construcciones y el pK7WG2-R como donador del gen de inter��s. Se logr�� obtener un vector intermediario pTNR-KAi-DsRed, con el cual se transformaron gen��ticamente las c��lulas quimiocompetentes de E.coli DH5��. Se obtuvieron ocho colonias de E. coli DH5�� transformadas. Se logr�� insertar el casete Pnit-DsRed-thcA HindII del vector intermediario en el sitio HindIII del MCS (Sitio M��ltiple de Clonaci��n por sus siglas en ingl��s) nuevamente en el pl��smido pTNR-KA para obtener el vector definitivo de transformaci��n de C924. Mediante la secuenciaci��n del casete de expresi��n Pnit-DsRed-thcA de 1,1 Kb se comprueba la existencia del promotor Pnit, del terminador thcA y el gen DsRed. Se estableci�� una metodolog��a de transformaci��n para la cepa C924 y se obtuvo expresi��n de la prote��na fluorescente recombinante.

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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