The subfamily Alysiinae (Hymenoptera, Braconidae) is one of the most peculiar and diverse endoparasitoids of the family Braconidae. A sampling survey was done in different parts of Iran, mainly in South, Central and South Eastern provinces. Fifteen species are recorded for the first time from Iran. They were belonging to tribe Alysiini (Dinotrema amparoae Peris-Felipo, 2013; D. borzhomii Tobias, 2004; D. dimorpha (Fischer, 1976); D. perlustrandum (Fischer, 1973); D. sinecarinum (Fischer, 1993); D. tauricum (Telenga, 1935); Orthostigma longicorne Konigsmann, 1969; Phaenocarpa bicolor (Foerster, 1863); P. carinthiaca Fischer, 1975 and Synaldis maxima Fischer, 1972) and Dacnusini (Chorebus melanophytobiae Griffiths, 1968; C. thusa (Nixon, 1937); Dacnusa areolaris (Nees, 1811); D. aquilegiae Marshall, 1896 and D. metula (Nixon, 1954)). List of Iranian Alysiinae including 108 species is also presented.