Data underlying the figures in the publication “Correlating single-molecule rupture mechanics with cell population adhesion by yeast display”, published in Biophysical Reports, 2022, Volume 2, Issue 1, 100035, ISSN 2667-0747. https://doi.org/10.1016/j.bpr.2021.100035 Table of contents: 1. Fig2_SMFS.xlsx: Contour Length vs probability density values for Figure 2D. Rupture Force and Loading rate values for all SMFS curves analysed for Figure 2E and 2F. 2. Fig3_SDA.xlsx: Shear Stress vs Cell Density values of all replicates and speeds for Figures 3D and 3E. 3. FigS1_SPR curves.xlsx: SPR curves reported in Supplementary Figure 1. 4. FigS2_one step FLN.xlsx: Force vs Extension trace and Contour Length Histogram values for one step FLN unfolding shown in Supplementary Figure 2. 5. FigS3_SMFS_internal control.xlsx: Rupture Force and Loading rate values for N-S25H reported in Supplementary Figure 3. 6. FigS5_SDA_internal control.xlsx: Shear Stress vs Cell Density values of N-WT shown in Supplementary figure 5B. 7. FigS6_SDA_induction timepoints.xlsx: Shear Stress vs Cell Density values and median of expression detected by flow cytometry reported in Supplementary Figure 6.