Background: Despite the well-known increased risk of exposure to the Covid-19 virus in a dental setting, vaccination rates among staff members are low. This study was aimed at understanding the knowledge, attitudes and perceptions of staff towards the Covid-19 vaccine. This information, as well as the possible associations to demographic profiles, are necessary for authorities to adequately address specific concerns and uncertainties; (2) Methods: A descriptive cross-sectional study was conducted by means of an anonymous, online, validated questionnaire.; (3) Results: 105 staff members participated. Majority of staff have received the Covid-19 vaccine but stated that they would not take the booster vaccination. Significant associations between the level of education and the knowledge, attitudes and perceptions of staff were found.; (4) Conclusions: Majority of the staff members had a positive attitude towards the Covid-19 vaccine. However, specific concerns and uncertainties were identified and will need to be addressed in order to improve vaccination rates among staff members.