School of Applied Science, Haldia Institute of Technology, Haldia-721 657, West Bengal, India bDepartment of Chemistry, Jadavpur University, Kolkata-700 032, India Department of Chemistry, Utkal University, Bhubaneswar-751 004, Odisha, India S-Inova Biotech, Programa de P��s-Gradua����o em Biotecnologia, Universidade Cat��lica Dom Bosco, Campo Grande, Brazil Centro de An��lises Prote��micas e Bioqu��micas, Programa de P��s-Gradua����o em Ci��ncias Gen��micas e Biotecnologia, Universidade Cat��lica de Bras��lia, Bras��lia, Brazil Department of Chemistry, School of Basic Science, SRM University Sikkim, Gangtok-737 102, Sikkim, India E-mail: gourisankar1978@gmail.com, rana_bidyut@yahoo.com, joydevdinda@gmail.com Manuscript received online 02 December 2020, revised and accepted 27 December 2020 A new Ru-N-heterocyclic carbene (Ru-NHC) complex (3a), chloro(p-cymene)-1-methyl-3-pyrimidylimidazolideneruthenium(II)- hexafluorophosphate was synthesized from a non-annulated NHC precursor,1-methyl-3-pyrimidylimidazoli um��hexafluorophosphate (3). The half-sandwich geometry of the molecule is established with single crystals X-ray diffraction. The absorption spectra and electrochemical nature of the complex are also investigated, supported by DFT analysis. Compound 3a is applied on lung (A549), colon (HCT116), and breast (MCF7) cancer cells to examine their in vitro cytotoxicity activity, and results are summarized and compared with a recent report for Ru(II)-NHC complexes containing annulated NHC ligands.