SOP for metabolite profiling of seminal plasma via GC Orbitrap

Seminal plasma chemical profiles provide distinct and metabolic phenotypes suited for fertility study monitoring. Profiling via GC-MS is favourable as it provides robust measures across many core primary metabolic pathways; including amino acids, TCA metabolites, carbohydrates and some lipids (e.g. steroids & fatty acid). A protocol for simple preparation of seminal plasma samples intended to undergo GC-MS analysis is described and enables large-scale semi-quantitative profiling.

Research Infrastructure RECETOX RI (No LM2018121) financed by the Ministry of Education, Youth and Sports, and Operational Programme Research, Development and Innovation - project CETOCOEN EXCELLENCE (No CZ.02.1.01/0.0/0.0/17_043/0009632).

Related Organizations

Masaryk University
Czech Republic

Keywords

metabolite profiling, eminal plasma, GC Orbitrap

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