The major data for “Changes in the Kuroshio path, surface velocity and transport during the last 35,000 years” are listed as follows: 1) The annual mean surface current (0-100m, m/s) in 5 cases. Data are separately saved in MATLAB files (surface_velocity_0ka.mat, surface_velocity_6ka.mat, surface_velocity_lgm.mat, surface_velocity_30ka.mat and surface_velocity_35ka.mat) with variables of longitude, latitude, u and v. 2) The Kuroshio paths (longitude and latitude) in 5 cases are saved in MATLAB file of “data_pathxy.mat”. 3) Distributions of upper 1000-m integrated volume-transport stream function from model results in 5 cases. Data is saved in the MATLAB file of “VT_model.mat” with variables of longitude, latitude, VT_model and casename. 3) Distributions of wind-driven Sverdrup transport stream function calculated by wind stresses in 5 cases. Data is saved in the MATLAB file of “ST_wind.mat” with variables of longitude, latitude, ST_wind and casename.