This dataset contains the raw and processed images from a low-cost high-throughput plant phenotyping (HTP) system, as well as the raw and processed images that were manually acquired for comparison. The HTP images were automatically and wirelessly acquired for entire benches of plants with a system composed of a Raspberry Pi and eight GoPro cameras. The entire file system of each GoPro camera was copied directly into a subfolder of finalGoProImages (numbered by camera). The raw HTP images were processed by correcting for lens distortion, computing the "greenness index" for each individual pixel, and filtering out extreme high and low values. These processed HTP images were then saved in the "greenness" subfolder of finalGoProImages. The manually acquired images in the finalDSLR folder each represent an individual plant from one of five time points during the same greenhouse experiment. The raw manually acquired images were processed in the same manner as the raw HTP images by computing the greenness index for each individual pixel and filtering out extreme high and low values. The two tab-delimited text files include the number of green pixels and mean greenness index for each HTP (greennessGoProTable2.txt) and manually acquired (greennessDSLRTable2.txt) image.