title : Reconstruction of hippocampus CA1 cell morphologies specimen : Mus musculus sex : male strain : C57BL/6J age : post-natal day 13-16 This neuron was recorded and filled with biocytin (3 mg/ml) in a 300 µm thick coronal slice of rat hippocampus, using 2 - 10 MOhm patch pipettes. 3,3′-diaminobenzidine (DAB) was used for revelation. The slice was fixed and the cell reconstructed with Neurolucida using a 100x oil immersion objective.