Image analysis pipelines from the article "AP-4-mediated axonal transport controls endocannabinoid production in neurons", published in Nature Communications by Davies et al. DAGLB_vs_TGN46_analysis_HeLa_cells_v3.1.9.cppipe - Cell Profiler (version 3.1.9) script used to analyse data shown in Fig. 2a, b and S2a, b. DAGLB_vs_TGN46_analysis_patient_neurons_v3.1.9.cppipe - Cell Profiler (version 3.1.9) script used to analyse data shown in Fig. 6a, b. DAGLB_vs_TGN46_analysis_SH-SY5Y_neuronal_cells_v3.1.9.cppipe - Cell Profiler (version 3.1.9) script used to analyse data shown in Fig. 2c, d and S2c, d. spot_colocalization_analysis.ijm - ImageJ (version 2.1.0) script used to analyse data shown in Fig. 5 and S4. High-throughput confocal imaging_MetaXpress_settings.docx - settings used for MetaXpress (version 6.7.0.211) to analyse data shown in Fig. 6c, e, f and S5b. Neurite outgrowth assays_IncuCyte_settings.docx - settings used for the Incucyte® Neurotrack Analysis Software Module in IncuCyte Controller (version 2020B) to analyse data shown in Fig. 7e-h, S6b-d and S7a-f. See article for methods and further detail.

This work was funded by the German Research Foundation (DFG/Gottfried Wilhelm Leibniz Prize MA 1764/2-1) and the Max Planck Society for the Advancement of Science. A.K.D. received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement no. 896725 and a Humboldt Research Fellowship. D.E.-F. had support from the CureAP4 Foundation, the Spastic Paraplegia Foundation, the National Institute of Health / National Institute of Neurological Disorders and Stroke (2R25NS070682 & 1K08NS123552-01) and the National Institutes of Health (BCH IDDRC, 1U54HD090255). M.Z. received scholarships from the DAAD (German National Exchange Service) and the German National Academic Foundation. J.E.A. is supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) 270949263/GRK2162, the DAAD (German National Exchange Service), the German National Academic Foundation and the Max Weber-Program of the State of Bavaria.