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yuan_t3ss_raw_data.zip: Microscopy data types: - Brightfield images - Fluorescence images (514 nm excitation) Microscope: Olympus IX-83 with 1.49NA oil-immersion objective (Olympus UAPON 100x) ET442/514/561 Laser triple band set filtercube (69904, Chroma) Camera: Hamamatsu C9100-13 EM-CCD Camera 80 nm pixel size yuan_t3ss_cell_objects.zip: EPEC and C41 ColiCoords cell objects derived from the raw data. The set contains a total of 24979 individual cells (brightfield, binary, fluorescence and localization datasets for each cell) with corresponding coordinate systems. Code to generate these cell objects from the raw data: https://github.com/Jhsmit/T3SS-paper The data format is .hdf5 and can be read with any HDF5 reader or directly with ColiCoords: https://github.com/Jhsmit/ColiCoords Contact: Jochem Smit
Fluorescence microscopy, EPEC, Live-cell microscopy
Fluorescence microscopy, EPEC, Live-cell microscopy
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