Virus induced gene silencing (VIGS) is a useful tool in plant biology to systemically silence a gene by RNA interference (RNAi). Co-expression of tobacco rattle virus 1 (TRV1), which contains the genome of the viral vector, with TRV2 containing the target sequence for RNAi, can be achieved by Agrobacterium-mediated gene expression in Nicotiana benthamiana (Burch-Smith et al., 2006). Existing methods of cloning TRV2 plasmids with the targeting sequence of interest include time consuming Restriction-Ligation cloning, expensive Gateway cloning or Ligation Independent Cloning (LIC). Whilst LIC is relatively quick and cost-effective, it relies on having a pre-linearised vector. Therefore, Golden-Gate cloning is preferable in that it is a 1-step cloning method. Therefore, we modified the existing TRV2 vector to be compatible with golden gate cloning. To add extra flexibility, we incorporated the overhangs used for pRNAiGG, a vector for carrying out transient, local silencing in leaf tissue by hairpin RNAi (Yan et al., 2012). Therefore, the resulting TRV2gg is compatible with the same insert for pRNAiGG enabling transient, local silencing or systemic silencing. TRV2gg contains an unwanted BsaI recognition site in the backbone, however a simple golden gate protocol of 37˚C for 2 h or a protocol that ends with a ligation-optimised step, is sufficient to easily clone multiple positive colonies.