Hyalinobatrachium tricolor sp. nov. (Fig. 11) Hyalinobatrachium nouraguense Lescure and Marty, 2000: 74. Hyalinobatrachium aff. iaspidiense Guayasamin et al., 2008a: 580. Holotype. MNCN 44827 (field code MB 250), adult male from Crique Wapou (04°26' N, 52°9' W; 2 m), Kaw, French Guiana, collected by M. Blanc on April 11, 2005. Paratopotypes. MNHN 2011.0116 (field code MB 247), MTD 48141 (field code MB 249), adult males collected by M. Blanc on April 4 and 11, 2005, respectively. Paratype. MNCN 44828 (field code MB 326), adult male from Kaw Mountain (04°32' N, 52°13' W; 10 m), French Guiana, collected by M. Blanc on January 18, 2008. Diagnosis. (1) Dentigerous processes on vomer and vomerine teeth absent; (2) snout truncated in dorsal and lateral view; (3) tympanum covered by skin, not visible through skin; (4) dorsal skin shagreened in life and preservative, (5) presence of small cloacal enameled warts; (6) parietal peritoneum transparent, pericardium transparent, visceral and hepatic peritonea white, all other peritonea transparent; (7) liver bulbous; (8) humeral spine absent; (9) webbing formula of fingers III 2 – (2 – 2+) IV; (10) webbing formula of toes I 1 – 2 II 1 – 2 III 1 – 2 IV 2 – 1 V; (11) enameled ulnar and tarsal folds; (12) nuptial pad Type-V, composed by a group of packed glands and situated in the medial, dorso-lateral internal side of Finger I, glands not present in other fingers, prepollex not evident from external view; (13) Finger I longer than Finger II; (14) eye diameter larger than width of disc on Finger III; (15) coloration in life: dorsum light green with big irregular darker green patches, brown warts, and minute melanophores, bones white; (16) coloration in preservative: cream with big irregular white patches and black dots; (17) iris yellow with dark grey flecks; (18) minute melanophores not extending throughout fingers and toes except base of Finger IV and Toe V; in life, tip of fingers and toes white; (19) advertisement call composed by four tonal notes but with a fast increase of energy at the beginning of each note lasting 0.19– 0.20 s, dominant frequency of 4628.11–4903.07 Hz, males call from the underside of leaves; (20) fighting behavior unknown; (21) egg clutches deposited on the underside of leaves, number of eggs per clutch 22 (N = 2), no parental care observed; (22) tadpole unknown; (23) medium size adult males, SVL = 20.3–21.0 mm (N = 4), females unknown. Comparisons. Following Guayasamin et al. (2009), we placed the new species in the genus Hyalinobatrachium because of the following characters: humeral spine absent in adult males; digestive tract and bulbous liver covered by white peritonea; completely transparent ventral parietal peritoneum; white bones in life; dorsal coloration cream in preservative; males lack conspicuous dorsal spinules during breeding season; nuptial pad small and restricted to the inner edge of Finger I in males (Type V); dentigerous process of the vomer and vomerine teeth absent; males vocalize from the undersides of leaves, and females deposit one layer of eggs on the undersides of leaves. The following unique combination of phenotypic characters differentiates Hyalinobatrachium tricolor sp. nov. from all other species in the genus: bones white in life, coloration in life lime green patches over a light green dorsum and brown warts, and advertisement call composed by four tonal notes lasting 0.19– 0.20 s and a dominant frequency of 4628.11–4903.07 Hz. Additional morphological, acoustic and genetic evidence supporting the status of this species could be found in Tables 1, 3, 5, and Figs 2, 3, 11. Nonetheless, in preservative is, to the best of our knowledge, indistinguishable from H. iaspidiense and H. mesai. (number of calls = 16). Time is given in seconds and frequency in Hertz, range is followed by mean and standard deviation. First note Note duration 0.015–0.025; 0.018 ± 0.003 Dominant frequency 4706.76–4859.01; 4761.327 ± 50.859 Lower frequency 3840–4232; 3981.125 ± 113.933 Upper frequency 5032–5290; 5116.875 ± 69.894 Time between notes 0.018–0.03; 0.025 ± 0.003 Second note Note duration 0.011–0.019; 0.015 ± 0.003 Dominant frequency 4635.73–4935.13; 4790.738 ± 86.326 Lower frequency 3248.000–4075.000; 3813.688 ± 211.981 Upper frequency 5183.000–5635.000; 5372.000 ± 139.295 Time between notes 0.049–0.550; 0.116 ± 0.170 Third note Note duration 0.008–0.016; 0.011 ± 0.002 Dominant frequency 4672.220–4903.070; 4804.573 ± 75.012 Lower frequency 3150.000–4094.000; 3800.438 ± 265.126 Upper frequency 5290.000–5936.000; 5597.375 ± 175.162 Time between notes 0.061–0.660; 0.105 ± 0.148 Fourth note Note duration 0.008–0.110; 0.028 ± 0.036 Dominant frequency 4732.140–4935.130; 4816.679 ± 69.292 Lower frequency 3023.000–4251.000; 3844.250 ± 316.712 Upper frequency 5290.000–5976.000; 5668.688 ± 185.800 Remarks. Lescure and Marty (2000) cited H. iaspidiense (= nouraguense) for Kaw, although they did not refer to any museum material. We assume that those specimens belong to H. tricolor sp. nov. Description of holotype. Adult male of small size, SVL 20.3 mm; head wider than body, HW 41 % of SVL; head wider than long (HW/HL = 1.3); snout truncate in dorsal view and profile; ES/EL = 0.8 and ES/IOD = 1.2; loreal region concave; nostrils prominent, round; internarial region depressed; canthus rostralis defined; eyes small, directed antero-laterally; EL 50 % of HL; tympanic annulus indistinct, tympanic membrane absent, supratympanic fold absent; dentigerous processes on vomers absent; choanae circular, separated; tongue elongate, ovoid, not attached to mouth posteriorly for about one sixth of its length; vocal slits extending from the sides of the base of tongue to the level of the mandibular joints. Forearms slim; diameter of forearms about one and a half times the diameter of upper arms; enameled ulnar fold evident; humeral spine absent; relative length of fingers: II

Published as part of Castroviejo-Fisher, Santiago, Vilà, Carles, Ayarzagüena, José, Blanc, Michel & Ernst, Raffael, 2011, Species diversity of Hyalinobatrachium glassfrogs (Amphibia: Centrolenidae) from the Guiana Shield, with the description of two new species, pp. 1-55 in Zootaxa 3132 on pages 33-36, DOI: 10.5281/zenodo.200895