Expanded fish mortality because of contaminations has constrained most ranchers to resort the utilization of chemotherapeutic operators particularly anti-microbials. The proceeded with utilization of these medications in aquaculture is getting to be unsafe as pathogens create obstruction and deduces unpredicted long haul general wellbeing impacts. More research endeavors are working to recognize elective sickness anticipation techniques, among which the utilization of probiotics has been proposed. The motivation behind this investigation was to detach and distinguish lactic acid bacteria as potential probiotics from Channa punctata (Taki fish) digestive tract. To absolute best of our insight, this is the primary methodology of detaching probiotic from Channa punctata. The absolute bacterial check was 2.64×1014cfu/g in the digestive system. The secluded strain was bar molded, gram positive, non-spore forming and along these lines affirmed as Lactobacillus. This strain showed significant development at pH 1, 2 and 3 and demonstrated to be acid tolerant; while it made due at 0.10% 0.15%, 0.20% and 0.25% bile salt fixation and they were found to tolerate in gastric juice. Result says it could be a potential probiotic since it would support in the antagonistic condition of fish digestive system and isolate have no any hostile impact. The isolate showed resistance to erythromycin, cefotaxime, chloramphenicol and antagonistic effect on Pseudomonas pathogen. Through disk diffusion method, 5.2×1013cfu/ml and 1.04×1014cfu/ml concentrated disk made of direct bacterial broth culture showed 20mm and 24mm zone respectively against Pseudomonas.