Abstract��� A preliminary chemical characterization of main components of toluene extract with dried rhizomes of Smilax domingensis Willd. that grow in Cuba was done using a GCMS-QP2010 Ultra Shimadzu and the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) libraries. After sample derivatization 893 chemical compounds were registered by the equipment and from them, 30 different chemical components were characterized and reported for the first time from this part of the plant in our country. The results demonstrate that the developed method could be employed as a rapid and versatile analytical technique for identification of chemical constituents and quality control of Smilax domingensis.