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Based on published records and new materials, we confirm presence of 110 ant species from the Dodecanese. The fauna of ants of the Dodecanese is distinct in relation to the other Greek provinces. Six species are insular endemics, and 19 species or morphospecies are known only from this archipelago. Four species are recorded from the Dodecanese for the first time. Additionally, Bothriomyrmex jannonei Menozzi, 1936 is synonymized with Bothriomyrmex communista Santschi, 1919, new synonymy.