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In total, 77 ant species collected from 40 sampling sites were recorded from the Western Greece administrative region, including 76 from the Achaia regional unit of Peloponnese and 31 from the Aetolia-Acarnania regional unit of Sterea Ellas. Myrmecocystus cursor var. hellenicus Forel, 1886 is restored from synonymy with Cataglyphis aenescens (Nylander, 1849) and proposed as Cataglyphis hellenica (Forel, 1886) bona species. Crematogaster lorteti Forel, 1910, Lasius jensi Seifert, 1982, Temnothorax tauricus (Ruzsky, 1902) and Tetramorium chefketi Forel, 1911 are recorded as new for Peloponnese, while presence of Ponera testacea Emery, 1895, Temnothorax morea Csösz, Salata & Borowiec, 2018 and Temnothorax tauricus (Ruzsky, 1902) was confirmed for Sterea Ellas. We found eight morphospecies not assigned to any formally described taxon which require further investigation as they can represent undescribed species. An updated list of ant species known from the Peloponnese province and Aetolia-Acarnania unit is given.