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Although synthetic dyes are commonly used, natural dyes are still being utilized and used to improve their intrinsic aesthetic properties as the main material for the body’s beauty. For example, research results have shown that henna plant leaves comprise dye together with other additives. This provides a hint that if color from henna is properly studied, it can be used not only as body decoration but may also have fiber-substrates affinity. This paper explores the dyeing possibility-the ability to dye and the fastness qualities of henna dye extracted from henna leaves on cotton fabric compared to reactive dyeing using the same dyeing technique as reactive dyeing. Also, color fastness tests have been performed according to the ISO test methods. The implications of henna dye have been shown to have poor to moderate dyeing capability towards cotton fabrics as opposed to the reactive dyes when henna dyeing is accompanied by reactive dyeing. Similarly, henna dye demonstrated satisfactory properties of fastness as opposed to reactive dye. For henna dye with 50% shade, it gives an outstanding color tone with a good level of coloration. Taken into account the ability to dye and the fastness of color, the dyeing of matured henna leaves is equally advantageous to the dyeing of cotton fabrics.