The diagrams of GC content and Nanopore reads depth, SNP number and Nanopore reads depth, and analyses of co-linearity against Nipponbare reference genome in 251 accessions.

Syntenic dot plots were the completeness of assembly sequences, and the Nanopore reads coverage and the number of SNPs indicated that few, if any, false duplications were present. The average GC content and nanopore reads coverage showed only one independent cluster means little to no contamination.

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